Cyclo (Tyrosyl-Prolyl) Produced by Streptomyces sp.: Bioactivity and Molecular Structure Elucidation

ROFIQ SUNARYANTO; BAMBANG MARWOTO; LIESBETINI HARTOTO; ZAINAL ALIM MAS'UD; TUN TEDJA IRAWADI

doi:10.5454/mi.5.2.5

Authors

ROFIQ SUNARYANTO Department of Agro-Industrial Technology, Institut Pertanian Bogor
BAMBANG MARWOTO Center of Biotechnology, Badan Pengkajian dan Penerapan Teknologi
LIESBETINI HARTOTO Department of Agro-Industrial Technology, Institut Pertanian Bogor
ZAINAL ALIM MAS'UD Department of Chemistry, Institut Pertanian Bogor
TUN TEDJA IRAWADI Department of Chemistry, Institut Pertanian Bogor

DOI:

https://doi.org/10.5454/mi.5.2.5

Keywords:

antibiotic, cyclo(tyrosyl-prolyl), inhibitory concentration, bioactivity

Abstract

Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by Streptomyces sp. have been carried out. The antibiotic was produced by liquid culture using Streptomyces sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, ¹H NMR, ¹³C NMR, and ¹³C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC27853 , Staphylococcus aureus ATCC25923, and Bacillus subtilis ATCC66923 were 27.0, 68.7, 80.2, and 73.7 Î¼g mL^-1 , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol^-1 , and molecular formula was C₁₄H₁₆N₂O₃ . Elucidation of molecular structure using ¹HNMR, ¹³C NMR, and ¹³C DEPT NMRshowed that antibiotic was cyclo(tyrosyl-prolyl).