Bacteria Associated with Arbuscula Mycorrhizal Spores Gigaspora margarita and  Their Potential for Stimulating Root Mycorrhizal Colonization and Neem (Melia  azedarach Linn) Seedling Growth

SRI WILARSO BUDI; YENNI BAKHTIAR; NUNANG LAMAEK MAY

doi:10.5454/mi.6.4.6

Authors

SRI WILARSO BUDI Department of Silviculture Faculty of Forestry, Institut Pertanian Bogor , Campus IPB Darmaga. Bogor
YENNI BAKHTIAR Center of Biotechnology, Badan Pengkajian dan Penerapan Teknologi, Gedung 630 Kawasan Puspitek Serpong Tangerang Selatan 15314, Indonesia
NUNANG LAMAEK MAY Faculty of Forestry, Universitas Cendrawasih, Jalan Camp Wolker , Jayapura 99358, Papua, Indonesia

DOI:

https://doi.org/10.5454/mi.6.4.6

Keywords:

bacteria, arbuscular mycorrizal fungi, stimulation effects, root colonization, plant growth

Abstract

Four isolates (BGi1, BGi2, BGi3, and BGi4) bacteria were isolated from surface sterilized arbuscular mycorrhizal fungi (AMF) spores of Gigaspora margarita (Gm). Based on 16S rDNA analyses and phylogenetic tree, it was revealed that isolates BGi1, BGi3 and BGi4 belong to genus Bacillus, whereas BGi2 was very closeÂ to Bacillus megaterium EG 24. Enzymatic activity test showed that all four isolates had cellulase and protease activities; while one isolate (Bacillus sp. BGi4) also has pectinase activity in addition to the celulaseÂ and protease activities. Dual inoculation of Melia azedarch Linn roots by B. megaterium BGi2Â and AMF spores G. margarita enhanced mycorrhizal root colonization by 58.3 %. Combination of Bacillus sp. BGi1 and G. margarita increasedÂ height, diameter, shoot biomass, and root biomass ofÂ M. azedarch byÂ 353, 4.8, 4546, and 2810%, respectively,Â in comparison to the uninoculated control plant.