Purification and Characterization of Glucose Oxidase of Aspergillus niger IPBCC.08.610

ANINDA INDRIANI; LAKSMI AMBARSARI; DEWI SESWITA ZILDA

doi:10.5454/mi.12.2.2

Purification and Characterization of Glucose Oxidase of Aspergillus niger IPBCC.08.610

Authors

ANINDA INDRIANI Institut Pertanian Bogor http://orcid.org/0000-0002-2330-8118
LAKSMI AMBARSARI
DEWI SESWITA ZILDA

DOI:

https://doi.org/10.5454/mi.12.2.2

Keywords:

Aspergillus niger IPBCC.08610, characterization, glucose oxidase, purification

Abstract

Glucose oxidase was an enzyme which catalyzed Î²-D-Glucose to gluconic acid and hydrogen peroxide. Glucose oxidase from Aspergillus niger IPBCC.08.610 was isolated, purified and characterized. The enzyme was purified by ammonium sulphate precipitation and dialysis. The specific activity and yield of dialysis fraction were 19.766 U/mg and 4.744%. The optimum pH and temperature were 6 and 30^oC. The stability of enzyme at optimum pH and temperature was decreasing 50% at 25 minutes. The k_m and v_max values for enzyme were 27 mM and 0.986 U/mg.

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Published

2018-08-27

How to Cite

INDRIANI, A., AMBARSARI, L., & ZILDA, D. S. (2018). Purification and Characterization of Glucose Oxidase of Aspergillus niger IPBCC.08.610. Microbiology Indonesia, 12(2), 2. https://doi.org/10.5454/mi.12.2.2