Levels of TNF-α in PBMC (Peripheral Blood Mononuclear Cells) Induced by Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in vitro

FITHRIYAH SJATHA, OKTIVIA CHANDRA MUSTIKA, BETI ERNAWATI DEWI, TJAHJANI MIRAWATI SUDIRO

Abstract


Dengue infection is a global health problem with an increasing incidence every year and now endemic in more than 100 WHO countries. Dengue infection is caused by dengue virus (DENV) which is an RNA virus with positive single strand, with ±11kb genome size encoding 3 structural proteins, 7 non-structural proteins, and two Untranslated Region (UTR). NS1 protein is known to have a very important role in the development of severe DENV infection, by the direct effect causing host cells damage and indirect effect by activating immune response to induce the secretion of excess cytokines. Transfected CHO-K1 cells with recombinant pcNS1 plasmid was co-cultured with PBMC from healthy donor. 48 hours post co-cultured, cell supernatant was collected and TNF-α levels and NS1 recombinant were measured by ELISA. Recombinant NS1 protein was expressed in CHO-K1 mammalian cell line and able to induce TNF-α with higher levels compared to control. Recombinant NS1 protein expression was able to induce secretion of TNF-α with higher levels compared to control.


Keywords


dengue; NS1; TNF-α; CHO-K1 cell



DOI: https://doi.org/10.5454/mi.13.2.%25p

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