Five Unique Amino Acid Residues of Hemagglutinin (HA) Proteins of Swine Influenza A (H1N1) Detected in 2009 in Jakarta, Indonesia

Authors

  • ANDI YASMON Department of Microbiology, Faculty of Medicine University of Indonesia, Jl. Pegangsaan Timur 16 Jakarta 10320 Indonesia
  • YULIANTY MUHAYAR Department of Microbiology, Faculty of Medicine University of Indonesia, Jl. Pegangsaan Timur 16 Jakarta 10320 Indonesia
  • VIVI SETIAWATY National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
  • BETI ERNAWATI DEWI Department of Microbiology, Faculty of Medicine University of Indonesia, Jl. Pegangsaan Timur 16 Jakarta 10320 Indonesia
  • BUDIMAN BELA Department of Microbiology, Faculty of Medicine University of Indonesia, Jl. Pegangsaan Timur 16 Jakarta 10320 Indonesia
  • FERA IBRAHIM Department of Microbiology, Faculty of Medicine University of Indonesia, Jl. Pegangsaan Timur 16 Jakarta 10320 Indonesia

DOI:

https://doi.org/10.5454/mi.6.2.3

Keywords:

amino acid substitution, H1N1, hemagglutinin, influenza A, pathogenicity

Abstract

Nine HA genes of influenza A (H1N1) viruses originating from swine which were detected in 2009 in Jakarta, Indonesia, were characterized in this study. Nasopharyngeal and/or pharyngeal samples were extracted to obtain viral RNA genomes. Amplification of the HA segment was performed by using the reverse transcription-polymerase chain reaction (RT-PCR), and followed by nested PCR in cases of RT-PCR negative. DNA sequencing was performed by using eight overlapping primers. All the Jakarta strains were closely related to vaccine strain A/California/07/2009. Nine amino acid changes were found in the Jakarta strains, and 5 (P100S, S220T, G239D, R240Q, and I338V) of those were unique to all Jakarta strains with respect to strain A/California/07/2009 used to produce vaccine. An I338V substitution was detected in a cleavage site of HA and no amino acid changes were detected in potential sites for N-linked glycosylation. For seven sites (positions 131, 158, 160, 183, 187, 222, and 227) playing an important role in viral attachment to host receptor, none of the expected amino acid changes was detected; however, a S220T substitution close to amino acid 222 was found in all the Jakarta strains. All amino acid changes potentially affect the pathogenicity of the viruses and the efficacy of strain A/California/07/2009 in neutralizing the Jakarta strains.

Published

2012-06-21

How to Cite

YASMON, A., MUHAYAR, Y., SETIAWATY, V., DEWI, B. E., BELA, B., & IBRAHIM, F. (2012). Five Unique Amino Acid Residues of Hemagglutinin (HA) Proteins of Swine Influenza A (H1N1) Detected in 2009 in Jakarta, Indonesia. Microbiology Indonesia, 6(2), 3. https://doi.org/10.5454/mi.6.2.3

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