Profile of Mutation on Drug Resistance Mycobacterium leprae Isolates in Indonesia Collected During 2003-2011

Authors

  • RATNA WAHYUNI Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyorejo Kampus C Unair, Surabaya 60115, Indonesia
  • DINAR ADRIATY Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyorejo Kampus C Unair, Surabaya 60115, Indonesia
  • ISWAHYUDI ISWAHYUDI Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyorejo Kampus C Unair, Surabaya 60115, Indonesia
  • CITA ROSITA SIGIT PRAKOESWA Departement of Dermatology and Venereology , Faculty of Medicine, Universitas Airlangga; Jalan Prof Dr Moestopo 47, Surabaya 60131, Indonesia
  • INDROPO AGUSNI Departement of Dermatology and Venereology , Faculty of Medicine, Universitas Airlangga; Jalan Prof Dr Moestopo 47, Surabaya 60131, Indonesia
  • SHINZO IZUMI Leprosy Study Group, Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyorejo Kampus C Unair, Surabaya 60115, Indonesia

DOI:

https://doi.org/10.5454/mi.6.3.7

Keywords:

dapsone, drug resistance, mutation, Mycobacterium leprae, rifampicin

Abstract

Multidrug therapy (MDT) regiment has been used for leprosy all over the world for more than 20 years. Drug resistances of Mycobacterium have been reported from many areas. The resistance mostly occurred due to mutation on the gene coding protein targeted by anti-leprosy drugs. Two hundreds and seventy M. leprae isolates from some area in Indonesia were examined for studying the profile of mutation among isolates collected during 2003-2011. Drug resistance determining region of the folP1 gene and the rpoB gene was sequenced. The results showed 5 isolates of M. leprae harboured mutation only in the folP1 gene and another isolate harbored mutation in both the folP1 and rpoB gene. The point mutation in the folP1 gene that was found in 2 isolates occurred in codon 53 (ACC→GCC; Thr→Ala). Double point mutations on codon 53 that was found in two isolates were ACC→AGA (Thr→Arg) and ACC→AGG (Thr→Arg). The point mutation in the folP1 geneoccurred in codon 55 were found in two isolates were CCC→CTC (Pro→Leu) and CCC→CGC (Pro→Arg). Whereas mutation in the rpoB gene in one isolate occurred in codon 410 was GAT→TAT (Asp→Tyr). These mutations that altered the amino acids of the protein revealed that isolates of M. leprae were resistant to drug with variable profiles

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Published

2012-12-14

How to Cite

WAHYUNI, R., ADRIATY, D., ISWAHYUDI, I., PRAKOESWA, C. R. S., AGUSNI, I., & IZUMI, S. (2012). Profile of Mutation on Drug Resistance Mycobacterium leprae Isolates in Indonesia Collected During 2003-2011. Microbiology Indonesia, 6(3), 7. https://doi.org/10.5454/mi.6.3.7
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