Genus Diversity of Actinomycetes in Cibinong Science Center, West Java, Indonesia

Authors

  • YANTYATI WIDYASTUTI Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
  • PUSPITA LISDIYANTI Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
  • SHANTI RATNAKOMALA Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
  • GINA KARTINA Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
  • RONI RIDWAN Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
  • ROHMATUSSOLIHAT ROHMATUSSOLIHAT Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
  • NITA ROSALINDA PRAYITNO Research Center for Biotechnology,Indonesian Institute of Sciences (LIPI), Jalan Raya Bogor Km 46, Bogor 16911Indonesian
  • EVI TRIANA Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Jakarta-Bogor Km 46, Bogor 16911, Indonesia
  • NUNUK WIDHYASTUTI Research Center for Biology, Indonesian Institute of Sciences (LIPI), Jalan Raya Jakarta-Bogor Km 46, Bogor 16911, Indonesia
  • RASTI SARASWATI Indonesia Soil Research Institute, Departement of Agricultutre, Jalan Tentara Pelajar No 12, Bogor 16114, Indonesia
  • RATIH DEWI HASTUTI Indonesia Soil Research Institute, Departement of Agricultutre, Jalan Tentara Pelajar No 12, Bogor 16114, Indonesia
  • YULIN LESTARI Faculty of Mathematics and Sciences, Institut Pertanian Bogor, Dramaga Campus, Bogor. 16680, Indonesia
  • MISA OTOGURO NITE Biological Resource Center, National Instituite of Technology and Evaluation, Kazusakamatari, Kisarazu-shi, Chiba, Japan
  • SHINJI MIYADOH NITE Biological Resource Center, National Instituite of Technology and Evaluation, Kazusakamatari, Kisarazu-shi, Chiba, Japan
  • HIDEKI YAMAMURA NITE Biological Resource Center, National Instituite of Technology and Evaluation, Kazusakamatari, Kisarazu-shi, Chiba, Japan
  • TOMOHIKO TAMURA NITE Biological Resource Center, National Instituite of Technology and Evaluation, Kazusakamatari, Kisarazu-shi, Chiba, Japan
  • KATSUHIKO ANDO NITE Biological Resource Center, National Instituite of Technology and Evaluation, Kazusakamatari, Kisarazu-shi, Chiba, Japan

DOI:

https://doi.org/10.5454/mi.6.4.4

Keywords:

16S rRNA gene sequencing, actinomycetes, diversity, Indonesia

Abstract

Actinomycetes are microorganisms that play important role to support human health and  known as soil microorganisms. The aim of the research was to describe genus diversity of actinomycetes in Cibinong Science Center (CSC), West Java. Samples for isolation were soil and plant litters. The samples were air dried and ground. We employed isolation methods: dry heat (DH), sodium dodecyl sulphates-yeast extract (SDS-YE), rehydration and centrifugation (RC), and oil separation (OS). A total of 263 isolates of actinomycetes were isolated in CSC, in 2004-2006. Totally 58, 144, 50, and 11 isolates were isolated under each isolation methods, respectively. All isolates were identified using the 16S rRNA gene sequencing method. The results showed that the isolates were belonged to the family Kineosporiaceae, Micromonosporaceae, Nocardiaceae, Pseudonocardiaceae, Streptomycetaceae, Streptosporangiaceae, Mycobacteriaceae, Nocardioidaceae, Nocardiopsaceae, and Thermomonosporaceae. There were 23 genera under those families. Homology value of the isolates based on BLAST search using 16S rRNA gene sequence data as queries showed that 136, 91, 30, and 6 isolates were ≥99, 98, 97, and ≤96%, respectively, compared to the known sequence in data base. The later 6 isolates were interesting for further identification leading to new taxa. Recognized species of Streptomyces genera under the member of the Streptomycetaceae were dominant among other isolates.

Published

2013-03-26

How to Cite

WIDYASTUTI, Y., LISDIYANTI, P., RATNAKOMALA, S., KARTINA, G., RIDWAN, R., ROHMATUSSOLIHAT, R., PRAYITNO, N. R., TRIANA, E., WIDHYASTUTI, N., SARASWATI, R., HASTUTI, R. D., LESTARI, Y., OTOGURO, M., MIYADOH, S., YAMAMURA, H., TAMURA, T., & ANDO, K. (2013). Genus Diversity of Actinomycetes in Cibinong Science Center, West Java, Indonesia. Microbiology Indonesia, 6(4), 4. https://doi.org/10.5454/mi.6.4.4

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