The Prevalence and Subtype Distribution of Hepatitis C Virus Infection among Hemodialysis Patients in a Private Hospital in Surabaya, Indonesia

Authors

  • MOCHAMAD AMIN Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia
  • JUNIASTUTI . Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia
  • TAKAKO UTSUMI Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo 650-0017, Japan
  • YOSHIHIKO YANO Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo 650-0017, Japan
  • MOCHAMAD YUSUF Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department of Cardiology, Faculty of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia
  • MOCHAMMAD THAHA Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department of Internal Medicine Faculty of Medicine, Faculty of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia
  • PRIYO BUDI PURWONO Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia
  • RETNO HANDAJANI Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department of Biochemistry, Faculty of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia
  • SOETJIPTO . Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department of Biochemistry, Faculty of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia
  • HAK HOTTA Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo 650-0017, Japan
  • YOSHITAKE HAYASHI Center for Infectious Diseases, Kobe University Graduate School of Medicine, Hyogo 650-0017, Japan
  • MARIA INGE LUSIDA Institute of Tropical Disease, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia Department of Microbiology, Faculty of Medicine, Universitas Airlangga, Jalan Mayjen Prof Dr Moestopo 47, Surabaya 60131, Indonesia Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Universitas Airlangga, Jalan Mulyoreijo, Surabaya 60115, Indonesia

DOI:

https://doi.org/10.5454/mi.6.4.5

Keywords:

anti-HCV, hepatitis C virus, hemodialysis, subtypes, Surabaya

Abstract

The prevalence of hepatitis C virus (HCV) infection has been as high as approximately 80% in patients with maintenance hemodialysis in public hospitals in Indonesia. However, the prevalence in private hospitals has not been examined yet. The aim of  this study was  to  investigate  the prevalence of anti-HCV antibody and  the subtype distribution in patients with hemodialysis in a private hospital in Surabaya, Indonesia. Sera were obtained from 41 hemodialysis patients in a private hospital in Surabaya. The positive prevalence of anti-HCV antibody was carried out by  the enzyme-linked  immunosorbent assay (ELISA). Anti-HCV-positive sera were subjected  to  reverse transcription-PCR (RT-PCR) to detect HCV RNA and then direct sequencing. The HCV subtype was examined by phylogenetic analysis. Twenty five patients (61%) out of 41 were positive for anti-HCV antibody, and HCV-RNA was detected in 19 patients. The positive prevalence of anti-HCV antibody was 7.7% (one out of 13 patients) among patients who had undergone hemodialysis  for  less  than one year, whereas  it was 85.7%  (24 out of  28  patients)  among  patients who  had  undergone  hemodialysis  for  over  one  year.  Phylogenetic  analysis revealed HCV-1a  (52.6%) was  the most common  subtype,  followed by 1b  (15.8%), 1c  (15.8%), 2a  (5.3%), and 3k (5.3%). In conclusion,  the prevalence of HCV  infection among hemodialysis patients  in a private hospital was as high as  that  in general hospitals. The predominant subtype was HCV-1a, which  is  in accordance with  the previous studies  in general hospitals  in Surabaya,  Indonesia.

Published

2013-04-09

How to Cite

AMIN, M., ., J., UTSUMI, T., YANO, Y., YUSUF, M., THAHA, M., PURWONO, P. B., HANDAJANI, R., ., S., HOTTA, H., HAYASHI, Y., & LUSIDA, M. I. (2013). The Prevalence and Subtype Distribution of Hepatitis C Virus Infection among Hemodialysis Patients in a Private Hospital in Surabaya, Indonesia. Microbiology Indonesia, 6(4), 5. https://doi.org/10.5454/mi.6.4.5

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