Enzymatic and Acid Hydrolysis of Sago Starch for Preparation of Ethanol Production

Authors

  • ROFIQ SUNARYANTO Center of Biotechnology BPPT, Kawasan PUSPIPTEK Serpong, Tangerang Selatan 15314,Banten , Indonesia
  • BERTI HARIASIH HANDAYANI Deparment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jalan Raya Bandung- Sumedang KM 21 Jatinangor , Sumedang 45363, West Java, Indonesia
  • RATU SAFITRI Deparment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jalan Raya Bandung- Sumedang KM 21 Jatinangor , Sumedang 45363, West Java, Indonesia

DOI:

https://doi.org/10.5454/mi.7.2.4

Abstract

A series of studies on the hydrolysis of Sago starch for ethanol fermentation had been conducted. Hydrolysis of sago starch was carried out using sulfuric acid 2.5% and amylase(s) enzymes. The concentrations of sago starch used in this experiment were  5, 10, 15, 20, and 30% (w/v). The highest hydrolyzate  containing reducing sugar was used as substrate for ethanol fermentation by Saccharomyces cerevisiae FNCC 3012. The results indicated  that hydrolysis using 2.5% sulfuric acid for 120 min at 121 °C produced 6.6%  (w/v)  reducing sugar and hydrolysis using α-amylase and Dextrozyme DX produced more reducing sugar, 7% (w/v) and 17.1% (w/v), respectively. The fermentation of hydrolyzed sago starch by S. cerevisiae FNCC 3012 produced ethanol 7.98% (v/v).

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Published

2013-07-15

How to Cite

SUNARYANTO, R., HANDAYANI, B. H., & SAFITRI, R. (2013). Enzymatic and Acid Hydrolysis of Sago Starch for Preparation of Ethanol Production. Microbiology Indonesia, 7(2), 4. https://doi.org/10.5454/mi.7.2.4

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