The Role of Arbuscular Mycorrhizal Fungus (Gigaspora margarita) on Mercury and Nutrients Accumulation by Enterolobium cyclocarpum Seedlings

Authors

  • HANNA ARTUTI EKAMAWANTI Tropical Silviculture Major, Post-graduate School of Bogor Agricultural University, Jalan Lingkar Akademik Kampus IPB Darmaga, Bogor 16680, Indonesia Faculty of Forestry, Tanjungpura University, Jalan Imam Bonjol, Pontianak, Indonesia
  • YADI SETIADI Laboratory of Forest Biotechnology, Research Center for Biological Resources and Biotechnology, Bogor Agricultural University, Jalan Lingkar Akademik Kampus IPB Darmaga, Bogor 16880, Indonesia
  • DIDY SOPANDIE Department of Agronomy and Horticulture, Bogor Agricultural University, Jalan Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
  • DWI ANDREAS SANTOSA Laboratory of Biotechnology Soil and Environment, Department of Soil and Land Resources, Bogor Agricultural University, Jalan Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

DOI:

https://doi.org/10.5454/mi.7.4.5

Keywords:

arbuscular mycorrhizal fungus, Enterolobium cyclocarpum, Gigaspora margarita, mercury

Abstract

A river-sand culture experiment was conducted to investigate whether arbuscular mycorrhizal (AM) colonization influenced mercury (Hg) and nutrients accumulation, and whether AM fungus (AMF) Gigaspora margarita enhance host plant sengon buto (Enterolobium cyclocarpum) tolerance to Hg. Hg was applied as HgCl2 at different levels (375 and 750 µM) and added to the full strength of Hoagland's solution, then applied to seedlings in river-sands as growth media according to treatments.  The non-mycorrhizal and mycorhizal E. cyclocarpum roots took up Hg, but its translocation to the leaves was inhibited. AM inoculation decreased significantly Hg content of roots seedlings by 70.5% from non-AM inoculation seedlings. Mycorrhizae enhanced significantly Ca and Mg uptake in shoot by 1.29- and 1.27-fold higher than non-mycorrhizal seedlings, but not enhanced significantly P uptake. Based on the roots dry weight, the tolerance index of non-mycorrhizal or mycorrhizal seedlings treated with 750 µM Hg supply was > 50%. It indicated that the seedlings can tolerate up to 750 µM Hg added. Considering the possible differences in AMF response to Hg in polluted soil from the field, it is not yet clear if Gi. margarita could be applied for phytoremediation of Hg in contaminated sites. Therefore, more work needs to be done using AMF isolates to reveal the possible application in the management of Hg contaminated soils.

Published

2014-05-05

How to Cite

EKAMAWANTI, H. A., SETIADI, Y., SOPANDIE, D., & SANTOSA, D. A. (2014). The Role of Arbuscular Mycorrhizal Fungus (Gigaspora margarita) on Mercury and Nutrients Accumulation by Enterolobium cyclocarpum Seedlings. Microbiology Indonesia, 7(4), 5. https://doi.org/10.5454/mi.7.4.5