Exploration, Isolation and Quantification of β-carotene from Bacterial Symbion of Acropora sp.

Authors

  • NAELY K. WUSQY
  • LEENAWATY LIMANTARA
  • FERRY F KARWUR

DOI:

https://doi.org/10.5454/mi.8.2.3

Keywords:

β-carotene, Acropora sp., Erythrobacter flavus, HPLC, 16S rDNA

Abstract

In the microbial world, pigments are one of the most conspicuous traits. Marine bacteria associated with Acropora sp. collected from Taka Cemara, Karimunjawa Islands were screened for the production of a yellow pigment. The isolation of bacterial symbionts from Acropora sp. on Zobell 2216E medium resulted in one bacterium, KJ5, positively synthesized carotenoids. By reverse phase HPLC analysis, one peak of the pigment types was identified as a β-carotene peak which appeared at 60.24 min. Then, sample of the β-carotene was collected and identified according to their spectral characteristics and compared with the published data in different types of solvent. Based on the HPLC analysis, the total β-carotene contents were calculated by converting the broad absorption of β-carotene. Molecular identification of the bacterium KJ5 using 16S rDNA showed that bacterium KJ5 was closely related to Erythrobacter flavus with 96% homology value.

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Published

2014-06-10

How to Cite

WUSQY, N. K., LIMANTARA, L., & KARWUR, F. F. (2014). Exploration, Isolation and Quantification of β-carotene from Bacterial Symbion of Acropora sp. Microbiology Indonesia, 8(2), 3. https://doi.org/10.5454/mi.8.2.3

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Section

Articles
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            PKPTemplateManager.display(frontend/pages/article.tpl) % line  287, file: ArticleHandler.inc.php
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      PKPRouter._authorizeInitializeAndCallRequest(Array[2], Object:Request, Array[1], false) % line  231, file: PKPPageRouter.inc.php
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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php
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                                             SubmissionSearch._getMergedArray(Object:Journal, Array[1], null, null) % line  263, file: SubmissionSearch.inc.php
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            PKPTemplateManager.display(frontend/pages/article.tpl) % line  287, file: ArticleHandler.inc.php
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      PKPRouter._authorizeInitializeAndCallRequest(Array[2], Object:Request, Array[1], false) % line  231, file: PKPPageRouter.inc.php
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Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php
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                                                      SubmissionSearch._getMergedKeywordResults(Object:Journal, Array[4], , null, null, 500, 1) % line  194, file: SubmissionSearch.inc.php
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                                             SubmissionSearch._getMergedArray(Object:Journal, Array[1], null, null) % line  263, file: SubmissionSearch.inc.php
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            PKPTemplateManager.display(frontend/pages/article.tpl) % line  287, file: ArticleHandler.inc.php
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      PKPRouter._authorizeInitializeAndCallRequest(Array[2], Object:Request, Array[1], false) % line  231, file: PKPPageRouter.inc.php
   PKPPageRouter.route(Object:Request) % line  143, file: Dispatcher.inc.php
Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php
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            PKPTemplateManager.display(frontend/pages/article.tpl) % line  287, file: ArticleHandler.inc.php
         ArticleHandler.view(Array[1], Object:Request) % line  391, file: PKPRouter.inc.php
      PKPRouter._authorizeInitializeAndCallRequest(Array[2], Object:Request, Array[1], false) % line  231, file: PKPPageRouter.inc.php
   PKPPageRouter.route(Object:Request) % line  143, file: Dispatcher.inc.php
Dispatcher.dispatch(Object:Request) % line  281, file: PKPApplication.inc.php
PKPApplication.execute() % line   68, file: index.php