Oil Recovery Test Using Bio surfactant of Halo tolerant Bacteria Brevundimonas diminuta and Bhurkholderia glumae at variation of NaCl Salt Concentrations

Abstract

Oil recovery test has been done by using crude biosurfactant from Brevundimonas diminuta and Bhurkholderia glumae indigenous halo tolerant bacteria with the vatiation of NaCl salt concentration 0; 1.5; 3; 4.5; 6; and 7.5%. Oil recovery test was obtained by determining % TPH (Total Petrolem Hidrocarbon). The sample concentration was 28.19% TPH, it was extracted by using biosurfactant of  Brevundimonas diminuta and Bhurkholderia glumae bacteria, the optimal salitnity conditions were  at 3, 4.5% salt concentrations with the value oil recovery as much as 50.41, 69.97 % respectively. Oil components which extraction by biosurfactant were analyzed by using GC-MS (Gas Chromatography-Mass Spectrophotometry). The result from analyzes GC-MS could be concluded that bacteria Brevundimonas diminuta could dissolve hydrocarbon compounds short chain carbon atom at fraction <C₁₀–C₁₄ and long chain carbon atom at fraction >C_22. <C₁₀, C₁₁-C₁₄dan C₁₅-C₁₇  and Bhurkholderia glumae could dissolve hydrocarbon compounds short chain carbon atom at fraction <C₁₀–C₁₄ and long chain carbon atom at fraction >C₂₂ according to the retention time.