Lipase Activity Enhancement of KC4J Mutant from Oil Palm Waste Using Response Surface Method and Characterization.

Authors

  • Aris Indriawan Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
  • Trismilah . Agency for the Assessment and Application of Technology, LAPTIAB, Bld.610-612, PUSPIPTEK, South Tangerang, 15314, Indonesia.
  • Wibowo Mangunwardoyo Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia
  • Dadang Suhendar Agency for the Assessment and Application of Technology, LAPTIAB, Bld.610-612, PUSPIPTEK, South Tangerang, 15314, Indonesia.

DOI:

https://doi.org/10.5454/mi.16.1.1-12

Abstract

Lipase has an important role in industry. The KC4J mutated isolates from oil palm  waste had 100% similarity to  Aspergillus fumigatus strain RA204, a fungus known produce lipase. The study aimed  to increase lipase activity of  KC4J mutant through media optimization using Response Surface Methodology (RSM) and partial characterization. The three variables of media composition  (olive oil, soy flour, and pH were optimized using Central Composite Design (CCD). The lipase characterization measured the influence of pH, temperature and metal ions. The pH  tested on range  6 to 12, while the temperature variation tested on 30 to 70 °C. The metal ions tested were Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ with concentrations of 1 mM and 10 mM. The  production medium containing 1.25% of olive oil, 3.5% of soy flour and 7.5 pH resulting 11.25 U/mL of Lipase activity, which was higher than the previous media composition (10.00 U/mL). The results of CCD and quadratic analysis showed that the source of carbon, nitrogen and pH had an effect on lipase activity which showed R2 0.93. The optimum lipase activity  produced  at pH 6 and  on 60 °C, and  the lipase stable at pH 6-8 and  on 30-70 °C. All metal ions tested were able to increase  lipase activity with Ca 2+ ion gave  the highest result.

Keywords: Lipase, KC4J mutant, Central Composite Design, Oil Palm Waste.

Downloads

Published

2022-06-21

How to Cite

Indriawan, A., ., T., Mangunwardoyo, W., & Suhendar, D. (2022). Lipase Activity Enhancement of KC4J Mutant from Oil Palm Waste Using Response Surface Method and Characterization. Microbiology Indonesia, 16(1), 1–12. https://doi.org/10.5454/mi.16.1.1-12

Issue

Section

Articles