@article{BAKHTIAR_YAHYA_SUMARYONO_SINAGA_BUDI_TAJUDDIN_2010, title={Isolation and Identification of Mycorrhizosphere Bacteria and Their Antagonistic Effects Towards Ganoderma boninense in vitro}, volume={4}, url={https://jurnal.permi.or.id/index.php/mionline/article/view/106}, DOI={10.5454/mi.4.2.9}, abstractNote={<span style="font-family: "Times New Roman","serif"; font-size: 8pt; mso-fareast-font-family: Calibri; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA;">Basal stem rot caused by <em style="mso-bidi-font-style: normal;">Ganoderma boninense</em> is the most serious disease of oil palm (<em style="mso-bidi-font-style: normal;">Elaeis guineensis</em>) in Indonesia and it has caused major loss in palm oil production.<span style="mso-spacerun: yes;">  </span>Mycorrhizosphere bacteria offer possible advantages as biocontrol agents as they live and proliferate together with arbuscular mycorrhizal fungi, which have an ability to increase plant resistance against pathogens.<span style="mso-spacerun: yes;">  </span>A study was conducted to isolate mycorrhizosphere bacteria from spores of arbuscular mycorrhizal fungi and test their antagonistic effects against <em style="mso-bidi-font-style: normal;">G. boninense</em> <em style="mso-bidi-font-style: normal;">in vitro</em>.<span style="mso-spacerun: yes;">  </span>All bacterial isolates were identified based on 16S rDNA analysis and it revealed that eleven out of twenty mycorrhizosphere bacteria isolated were related to Bacillus with similarity ranging from 97 to 100%, whereas other isolates were identified as <em style="mso-bidi-font-style: normal;">Pseudomonas, Streptomyces,</em> <em style="mso-bidi-font-style: normal;">Kocuria, Enterobacter, Brevundimonas</em>, and <em style="mso-bidi-font-style: normal;">Alcaligenes</em> with similarities ranging from 96 to 100%.<span style="mso-spacerun: yes;">  </span>Fourteen out of twenty mycorrhizosphere bacteria showed a varying degree of inhibition towards the growth of <em style="mso-bidi-font-style: normal;">G. boninense in vitro</em>.<span style="mso-spacerun: yes;">  </span>Of these, isolate B10 (closely related to <em style="mso-bidi-font-style: normal;">Bacillus subtilis</em> ZJ06) showed the highest inhibitory effect followed by B17 (closely related to <em style="mso-bidi-font-style: normal;">Bacillus subtilis</em> N43).<span style="mso-spacerun: yes;">  </span>Therefore, these bacteria have a potential to be used as biocontrol agents to control basal stem rot disease caused by <em style="mso-bidi-font-style: normal;">G. boninense</em> in oil palm. </span>}, number={2}, journal={Microbiology Indonesia}, author={BAKHTIAR, YENNI and YAHYA, SUDIRMAN and SUMARYONO, WAHONO and SINAGA, MEITY SURADJI and BUDI, SRI WILARSO and TAJUDDIN, TEUKU}, year={2010}, month={Aug.}, pages={9} }