@article{NURKANTO_AGUSTA_SJAMSURIDZAL_JULISTIONO_2013, title={Bacterial Response after Exposure with Pure Metabolite Produced by Streptomyces sp. BL225 Isolated from Batanta Island’s Leaf Litter}, volume={7}, url={https://jurnal.permi.or.id/index.php/mionline/article/view/198}, DOI={10.5454/mi.7.1.4}, abstractNote={<p>The objective of this research was to investigate bacterial response after treatment with active metabolite produced by <em>Streptomyces</em> sp. isolated from Batanta Island. Minimum inhibitory concentration (MIC) values of four clinically tested bacteria (<em>Escherichia coli</em>, <em>Bacillus subtilis</em>, <em>Staphylococcus aureus</em>, and <em>Micrococcus luteus</em>) were successfully determined in this research using microdilution method. Leakages of nucleic acids and proteins from the tested microbes were detected using UV/VIS spectrophotometry method at 260 and 280 nm. Uracil leakage was analyzed using HPLC. Morphological changes of the bacterial cells were observed using scanning electron microscope (SEM). A Streptomyces isolate BL225 was identified based on the 16S rRNA gene sequence (1500 bp). When tested agains microbes, the MICs values of this compound were between 16-64 µg mL<sup>-1</sup>. The results indicated leakages of protein, nucleic acid and uracil from <em>E. coli</em> and <em>B. subtilis</em> cells after treatment with pure metabolite isolated from BL225. Treatment using metabolite from BL225 also caused morphological changes and damages of the target bacterial cell. BL225 had been identified as a strain that has closed relation to <em>Streptomyces badius</em> (98.9%).</p>}, number={1}, journal={Microbiology Indonesia}, author={NURKANTO, ARIF and AGUSTA, ANDRIA and SJAMSURIDZAL, WELLYZAR and JULISTIONO, HEDDY}, year={2013}, month={Mar.}, pages={4} }