@article{Optimization of Lipases Production byBacillus licheniformis F11.4 using Response Surface Methodology_2017, volume={10}, url={https://jurnal.permi.or.id/index.php/mionline/article/view/532}, DOI={10.5454/mi.10.4.4}, abstractNote={<p>Lipase is a lipids hydrolyze enzyme which are widely used in various industries such as chemical, pharmaceutical, food industries, and detergents. Bacillus licheniformis F11.4 is one of the bacteria with<br />potential source of lipase. This study aimed to obtain optimum production of lipase from B. licheniformis F11.4 by optimizing the composition of media and pH values with fish flour as a replacement for peptone and<br />yeast extract based medium. Selection of the significant factors used a 2-level factorial design. The upper limit and lower limit of the selected factors was optimized using Central Composite Design (CCD) and the<br />data analysis was performed using the Response Surface Methodology (RSM). Fermentation was carried out in erlenmeyer at initial pH 8 and a temperature of 37 °C, using a shaker incubator at 150 rpm. A fermentation system for lipases production is considered optimal when its desirability value closes to 1. By using numerical optimization, an optimal medium could be obtained, i.e. consisting of OO:CPO 0.14 % (w/v) and<br />fish flour 2% (w/v), at pH 8 and 150 rpm, which produced lipase with enzyme activity of 1.563 U mL-1 and protein level of 0.08 mg mL-1.Furthermore, the results are verified in the Erlenmeyer, working volume of 50 mL, pH = 8, T = 37 °C, agitation 150 rpm, t=18 hours, the activity of lipase and protein levels are 1.568 ± 0.014 U mL-1 and 0.072 ± 0.006 mg mL-1 respectively.The results showed that the optimum condition lipase<br />activity was 1.568 U mL-1 so that the increase in the activity of only 75% compared to before optimization.</p>}, number={4}, journal={Microbiology Indonesia}, year={2017}, month={Jun.}, pages={4} }