@article{Hallis_Hartanti_Gunawan_2021, title={Molecular Diversity of Mold Associated with Gatotan}, volume={15}, url={https://jurnal.permi.or.id/index.php/mionline/article/view/817}, DOI={10.5454/mi.15.1.1}, abstractNote={<p><em>Gatotan</em> is a raw material to make <em>gatot</em>, an Indonesian fermented food, especially in Central Java, made from cassava tubers. Traditionally, the cassava tubers are sun-dried for several days until the black color appears. However, natural fermentation allowed by many types of microorganisms, especially mold in this process could raise concerns about the food safety issues. Previously, the identifications of molds in <em>gatotan</em> were based on morphological observation. Here, we reported the diversity of molds associated with <em>gatotan</em> using molecular identification method. The molecular identification was based on ribosomal DNA internal transcribed spacer (ITS) amplification sequences using combination of ITS4 and ITS5 primers. A total of ten molds were isolated and phylogenetic trees were constructed based on ribosomal DNA sequences. Our results showed that the molds were classified into <em>Lasiodiplodia </em>spp., <em>Trichoderma</em> sp., <em>Aspergillus nomius, Fusarium</em> <em>oxysporum</em>, and <em>Cladosporium</em> <em>sphaerospermum. </em></p>}, number={1}, journal={Microbiology Indonesia}, author={Hallis, Steffanus Pranoto and Hartanti, Anastasia Tatik and Gunawan, Agustin Wydia}, year={2021}, month={Apr.}, pages={1} }