TY - JOUR AU - SUNARYANTO, ROFIQ AU - MARWOTO, BAMBANG AU - HARTOTO, LIESBETINI AU - MAS'UD, ZAINAL ALIM AU - IRAWADI, TUN TEDJA PY - 2011/10/16 Y2 - 2024/03/29 TI - Cyclo (Tyrosyl-Prolyl) Produced by Streptomyces sp.: Bioactivity and Molecular Structure Elucidation JF - Microbiology Indonesia JA - Microbiol indones VL - 5 IS - 2 SE - Articles DO - 10.5454/mi.5.2.5 UR - https://jurnal.permi.or.id/index.php/mionline/article/view/139 SP - 5 AB - <p>Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by <em>Streptomyces </em>sp. have been carried out. The antibiotic was produced by liquid culture using <em>Streptomyces</em> sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, <sup>1</sup>H NMR, <sup>13</sup>C NMR, and <sup>13</sup>C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to <em>Escherichia coli </em>ATCC 25922, <em>Pseudomonas aeruginosa</em> ATCC27853 , <em>Staphylococcus aureus</em> ATCC25923, and <em>Bacillus subtilis</em> ATCC66923 were 27.0, 68.7, 80.2, and 73.7 μg mL<sup>-1</sup> , respectively. Identification using ESI-MS showed that the molecular weight of this antibiotic was 260 g mol<sup>-1</sup> , and molecular formula was C<sub>14</sub>H<sub>16</sub>N<sub>2</sub>O<sub>3</sub> . Elucidation of molecular structure using <sup>1</sup>HNMR, <sup>13</sup>C NMR, and <sup>13</sup>C DEPT NMRshowed that antibiotic was cyclo(tyrosyl-prolyl).</p> ER -