TY - JOUR AU - KUSMINTARSIH, ENDANG SRIMURNI PY - 2012/12/14 Y2 - 2024/03/28 TI - Effects of Tetracycline and Temperature on Drosophila melanogaster Infected with Wolbachia Inducing the Popcorn-Effect JF - Microbiology Indonesia JA - Microbiol indones VL - 6 IS - 3 SE - Articles DO - 10.5454/mi.6.3.6 UR - https://jurnal.permi.or.id/index.php/mionline/article/view/181 SP - 6 AB - <p>The expression of cytoplasmic incompatibility, parthenogenesis, and feminization in many hosts of Wolbachia is influenced by environmental factors such as temperature and antibiotics. Therefore, it might also affect <em>Wolbachia</em> inducing popcorn-effect. To examine the effects of temperature and antibiotic treatment on the life span of <em>Drosophila melanogaster</em> harbouring the popcorn-effect inducing strain of <em>Wolbachia</em>, flies were reared in different temperature such as at 20 and 29 °C, and cultured (from egg to adult stage) on a medium containing tetracycline. The tetracycline-treated <em>Wolbachia</em> was established by placing 0.25 mg m L<sup>-1</sup> of tetracycline in the media. The result showed that there was no difference in the life span of <em>D. melanogaster</em> infected with <em>Wolbachia</em> popcorn-effect under untreated and treated condition with tetracycline at 20 °C. Therefore, there is no popcorn-effect in the <em>D. melanogaster</em> at low temperature (20 °C). While the life span of <em>D.</em> <em>melanogaster</em> at 29 °C, where infected flies have a shorter life span than treated flies. Popcorn-effect (shorter life span) was found at 29 °C.</p> ER -