TY - JOUR AU - NURKANTO, ARIF AU - AGUSTA, ANDRIA AU - SJAMSURIDZAL, WELLYZAR AU - JULISTIONO, HEDDY PY - 2013/03/31 Y2 - 2024/03/28 TI - Bacterial Response after Exposure with Pure Metabolite Produced by Streptomyces sp. BL225 Isolated from Batanta Island's Leaf Litter JF - Microbiology Indonesia JA - Microbiol indones VL - 7 IS - 1 SE - Articles DO - 10.5454/mi.7.1.4 UR - https://jurnal.permi.or.id/index.php/mionline/article/view/198 SP - 4 AB - <p>The objective of this research was to investigate bacterial response after treatment with active metabolite produced by <em>Streptomyces</em> sp. isolated from Batanta Island. Minimum inhibitory concentration (MIC) values of four clinically tested bacteria (<em>Escherichia coli</em>, <em>Bacillus subtilis</em>, <em>Staphylococcus aureus</em>, and <em>Micrococcus luteus</em>) were successfully determined in this research using microdilution method. Leakages of nucleic acids and proteins from the tested microbes were detected using UV/VIS spectrophotometry method at 260 and 280 nm. Uracil leakage was analyzed using HPLC. Morphological changes of the bacterial cells were observed using scanning electron microscope (SEM). A Streptomyces isolate BL225 was identified based on the 16S rRNA gene sequence (1500 bp). When tested agains microbes, the MICs values of this compound were between 16-64 µg mL<sup>-1</sup>. The results indicated leakages of protein, nucleic acid and uracil from <em>E. coli</em> and <em>B. subtilis</em> cells after treatment with pure metabolite isolated from BL225. Treatment using metabolite from BL225 also caused morphological changes and damages of the target bacterial cell. BL225 had been identified as a strain that has closed relation to <em>Streptomyces badius</em> (98.9%).</p> ER -