TY - JOUR AU - YUSRA, YUSRA AU - AZIMA, FAUZAN AU - NOVELINA, NOVELINA AU - PERIADNADI, PERIADNADI PY - 2014/03/21 Y2 - 2024/03/28 TI - Characterization of Antimicrobial Bacteriocin Produced by Bacillus cereus SS28 Isolates from Budu, a Traditionally Fermented Fish Product of West Sumatera JF - Microbiology Indonesia JA - Microbiol indones VL - 8 IS - 1 SE - Articles DO - 10.5454/mi.8.1.4 UR - https://jurnal.permi.or.id/index.php/mionline/article/view/266 SP - 4 AB - <p class="p8 ft16"><span class="ft14">Bacillus cereus </span>SS28 isolated from budu, a fermented fish product from West Sumatra, produced antimicrobial compound that had broad spectrum of inhibition against five microorganisms (<span class="ft14">Escherichia coli</span>, Staphylococcus aureus<span class="ft17">, </span>Salmonella thypi<span class="ft17">, </span>Bacillus subtilis<span class="ft17">, and </span>Listeria monocytogenes<span class="ft17">). The aims of this research are characterization of </span>Bacillus cereus <span class="ft17">SS28 antimicrobial activity and observation of its effect to the cellular morphology of </span>Staphylococcus aureus <span class="ft17">with electron microscope. Antimicrobial compound produced by </span>Bacillus cereus <span class="ft17">SS28 was stable at pH range between 2 and 11 and to heating at 121 </span><span class="ft17"><span class="ft17">°C</span> for 15 min. Maximum antimicrobial activity was expressed at pH </span><span class="ft17">2-3</span><span class="ft17"> and 70 °C for 45 min. The activity remained after 15 min exposure to UV light. The main changes observed under SEM and TEM were the alteration of </span>Staphylococcus aureus <span class="ft17">structural cell membrane 48 h after exposure to the antimicrobial compound from Bacillus cereus SS28.</span></p> ER -