TY - JOUR AU - NOVELNI, RINGGA AU - MARLINA, MARLINA AU - RAVEINAL, RAVEINAL PY - 2015/09/30 Y2 - 2024/03/29 TI - Determination of blaVIM and blaIMP Resistant Genes againts Meropenem of Pseudomonas aeruginosa Isolated from HCU Bronkopneumona Inpatients at Internal Medicine RSUP dr M Djamil Padang JF - Microbiology Indonesia JA - Microbiol indones VL - 9 IS - 3 SE - Articles DO - 10.5454/mi.9.3.5 UR - https://jurnal.permi.or.id/index.php/mionline/article/view/360 SP - 5 AB - In this research, we aimed to detect <em>bla<sub>VIM</sub></em> and <em>bla</em><em><sub>IMP</sub></em> the resistant genes against Meropenem in <em>Pseudomonas aeruginosa </em>from sample of hospitalization patients at the Internal Medicine HCU of RSUP DR. M. Djamil Padang. Firstly, bacterial isolate of <em>P. </em><em>aeruginosa </em>were isolated from the sputum samples of patients who suffered bronkopneumonia. The isolation were started with samples cultivation to the Cetrimide Agar media which was a selective media for <em>P. aeruginosa</em><em>. </em>To determine the species of the bacteria, the identification using Gram staining, Triple Sugar Iron Agar (TSIA) test, citric test, urease test, Methyl Red/ Voges–Proskauer (MR/VP) test, and molecular marker of <em>16S rRNA</em> genes have been conducted. The isolation and identification result showed that from 20 sputum samples of the patients there were just 10 (50%) samples were positively containing <em>P. </em><em>aeruginosa</em>. From the <em>P. aeruginosa</em><em> </em>isolates, the resistant genes against meropenem <em>bla<sub>VIM</sub></em> and <em>bla</em><em><sub>IMP </sub></em>were amplified using PCR. The result showed that all these <em>P. aeruginosa </em>isolates have positively genes encoding for Metallo-β-Lactamase (MBLs). ER -