TY - JOUR AU - EVELINE, EVELINE AU - TANUMIHARDJA, CHIKITA WINI PY - 2020/01/24 Y2 - 2024/03/29 TI - Antibacterial Potential of Radish Extract (Raphanus sativus L.) Against Fish Spoilage Bacteria JF - Microbiology Indonesia JA - Microbiol indones VL - 13 IS - 3 SE - Articles DO - 10.5454/mi.13.3.3 UR - https://jurnal.permi.or.id/index.php/mionline/article/view/719 SP - 3 AB - <p>Radish (<em>Raphanus sativus</em> L.) root is commonly used  as flavor enhancing additive or side dish. Previous research revealed the presence of active compound in which could inhibit bacterial growth. Thus, a research concerning natural antibacterial for fish products that are categorized as high-risk food being contaminated by spoilage bacteria (<em>Pseudomonas aeruginosa, Bacillus cereus, </em>dan<em> Staphylococcus aureus</em>) was done. Radish root extraction was made by using ethyl acetate (semi polar) for 3 days. Well diffusion was performed using 4 extract concentration (10, 20, 30, dan 40% (w/v)) againts  three fish spoilage bacteria. Based on our results, 30% concentration was the best concentration which inhibit more than 10 mm in inhibition zone with MIC  and MBC. The scores were of 0.06% and 0.24% (<em>P. aeruginosa</em>), 0.13% and 0.50% (<em>S. aureus</em>), and 0.12% and 0.48% (<em>B. cereus</em>). Moreover, based on stability test against heating temperature showed that this extract concentration</p><p>was more stable in 80°C with duration times for 5 minutes and pH 3 which resulting the lowest inhibition zone reduction compares to control extract. Radish extract was categorized as low toxic compound (LC<sub>50</sub> = 839.52 ppm) functioning in antibacterial compound containing major antibacterial compound<em> </em>(<em>bis(2-ethylhexyl) phthalate</em>, 1,2-<em>benzenedicarboxylic acid</em>, <em>9,12,15-octadecatrienoic acid</em>), fatty acid (<em>n-hexadecanoic acid</em>,<em> butanedioic acid</em>), carboxylic acid (<em>isobutyric acid</em>, <em>malic acid</em>,<em> oleic acid</em>), and minor antibacterial compound (<em>n-Hydroxymethylacetamide</em>,<em> 2,4-bis(1,1-dimethylethyl)</em>,<em> 2,4-pentanedione</em>,<em>2-Cyclohexen-1-one</em>,<em> hydrazine</em>,<em> cyclohexene oxide</em>,<em> gamma-sitosterol</em>).</p> ER -