Analyses of Precore and Core Promoter Mutations of Hepatitis B Virus in Patients with Chronic Hepatitis B in Surabaya, Indonesia

Authors

  • . JUNIASTUTI Department of Microbiology, School of Medicine, Universitas Airlangga
  • EDUARDUS BIMO AKSONO Institute of Tropical Disease, Universitas Airlangga
  • TAKAKO UTSUMI Center for Infectious Diseases, Kobe University Graduate School of Medicine
  • YOSHIHIKO YANO Center for Infectious Diseases, Kobe University Graduate School of Medicine
  • . SOETJIPTO Department of Biochemistry, School of Medicine, Universitas Airlangga
  • YOSHITAKE HAYASHI Center for Infectious Diseases, Kobe University Graduate School of Medicine
  • HAK HOTTA Center for Infectious Diseases, Kobe University Graduate School of Medicine
  • FEDIK ABDUL RANTAM Center for Infectious Diseases, Kobe University Graduate School of Medicine
  • HERNOMO ONTOSENO KUSUMOBROTO Department of Internal Medicine, Dr. Soetomo General Hospital
  • MARIA INGE LUSIDA Department of Microbiology, School of Medicine, Universitas Airlangga

DOI:

https://doi.org/10.5454/mi.4.3.%25p

Keywords:

hepatitis B virus, precore mutations, core promoter mutations, chronic hepatitis B

Abstract

Mutations of precore (A1896) and core promoter (T1762/A1764) of hepatitis B virus can reduce HBeAg production. These mutations are frequently found in the late HBeAg seroconversion. However, it has been a controversy about the role played by precore and core promoter mutations in determining outcome of chronic hepatitis B. In the present study, the variability of precore and core promoter of hepatitis B virus were analyzed using PCR amplification and sequencing, according to the outcome (viral load and HBeAg/anti-HBe) in chronic hepatitis B patients in Surabaya. The study groups included 5 patients with uncomplicated chronic hepatitis B and 10 patients with chronic hepatitis B and liver cirrhosis in Dr. Soetomo Hospital, Surabaya. The control group included 6 blood donors obtained from Indonesia Red Cross, Surabaya. All groups were HBsAg positive. Precore mutation A1896 was predominant in all groups (60%-67% of each), together with precore variant T1858. As reported, precore variant T1858 is a prerequisite for precore A1896 and characteristic for viral genotype. Nevertheless, core promoter mutations T1762/A1764 were predominant only in LC patients (60%). All of these mutations were found mostly after HBeAg seroconversion (anti-HBe+). Of most samples with anti-HBe+, precore mutation was related with low viral load (<105 copies/mL), but core promoter mutations with high viral load (>105 copies/mL). Precore mutation A1896 was predominant in all groups, but core promoter mutations T1762/A1764 were only predominant in LC patients. The precore mutation alone is possible not critical to indicate a poor outcome, the core promoter mutations must be considered also.

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How to Cite

JUNIASTUTI, ., AKSONO, E. B., UTSUMI, T., YANO, Y., SOETJIPTO, ., HAYASHI, Y., HOTTA, H., RANTAM, F. A., KUSUMOBROTO, H. O., & LUSIDA, M. I. (2010). Analyses of Precore and Core Promoter Mutations of Hepatitis B Virus in Patients with Chronic Hepatitis B in Surabaya, Indonesia. Microbiology Indonesia, 4(3). https://doi.org/10.5454/mi.4.3.%p

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