Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation
DOI:
https://doi.org/10.5454/mi.10.3.5Keywords:
Bacillus halodurans CM1, optimization production, RSM, xylanaseAbstract
A two level factorial design was performed to optimized xylanase production by alkalothermophilic Bacillus halodurans CM1 using response surface method. The variables involved in this experiment were carbon (X), 1 nitrogen source (X) concentration, and pH (XCorn cob and fish powder were use as carbon and nitrogen source 2 3 respectively. Statistical analysis of the experimental results in the range studied, only carbon source gave significant effect on xylanase production. Â A second-order model was proposed to represent the enzyme activity as a function of xylan concentration (X) and pH (X). Â The optimum corn cobs concentration was 4.37% (w/v), 1 3 fish powder P concentration was 1.75% (w/v) and pH 9. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, and the highest xylanase activity.Downloads
Published
2016-09-30
How to Cite
WIBOWO, S. G., HELIANTI, I., SURYANI, A., & WAHYUNTARI, B. (2016). Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation. Microbiology Indonesia, 10(3), 5. https://doi.org/10.5454/mi.10.3.5
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