Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation
Keywords:Bacillus halodurans CM1, optimization production, RSM, xylanase
AbstractA two level factorial design was performed to optimized xylanase production by alkalothermophilic Bacillus halodurans CM1 using response surface method. The variables involved in this experiment were carbon (X), 1 nitrogen source (X) concentration, and pH (XCorn cob and fish powder were use as carbon and nitrogen source 2 3 respectively. Statistical analysis of the experimental results in the range studied, only carbon source gave significant effect on xylanase production. A second-order model was proposed to represent the enzyme activity as a function of xylan concentration (X) and pH (X). The optimum corn cobs concentration was 4.37% (w/v), 1 3 fish powder P concentration was 1.75% (w/v) and pH 9. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, and the highest xylanase activity.
How to Cite
Copyright (c) 2017 Microbiology Indonesia
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.