Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia

Authors

  • AMARILA MALIK Faculty of Pharmacy, Universitas Indonesia
  • IMAN SANTOSO FMIPA,Universitas Indonesia
  • ANDI YEHUDA FMIPA, Universitas Indonesia
  • SERUNI K.U. FREISLEBEN FMIPA, Universitas Indonesia
  • SEPTELIA INAWATI WANANDI FK, Universitas Indonesia
  • HARALD HUBER Department of Microbiology, Archaea Centre, University of Regensburg, Germany
  • ZESSINDA LUTHFA FMIPA, Universitas Indonesia
  • ROSARI SALEH FMIPA, Universitas Indonesia
  • HANS-JOACHIM FREISLEBEN FK, Universitas Indonesia

DOI:

https://doi.org/10.5454/mi.8.1.3

Keywords:

Indonesian volcanoes, Tangkuban Perahu, tetraether lipid, Thermoplasma

Abstract

Archaea is an organisme with unique feature because of its ability to inhabit an extremophyle conditions. Our expeditions to Tangkuban Perahu, West Java aimed to obtain archaealstrains from the solfatara fields located in Domas crater. From the samples, we intended to culture Thermoplasma species growing around 55 °C below pH 2, which until now have not yet been fully characterized. We collected five samples from mud holes with temperatures from 52 °C to 57 °C and pH below 2. In serial cultures of up to 8 transfers in Freundt’s medium we grew tetraetherlipid synthesizing Thermoplasma species as documented by phase contrast microscope. Total membrane lipid extracts were analysed by thin layer chromatography; the pattern matched total lipid extracts from Thermoplasma acidophilum DSM 1728 membranes. For confirmation, 16S rDNA identification performing PCR and sequencing were carried-out. Analysis using BLAST showed T. acidophilum identities as the highest similarity of 99%, followed by T. volcanium, also with99% similarity (ANKF776908 and ANKF776909). This is the first report of culturing cell-wall-less thermoacidophilicarchaea,in particular Thermoplasma species in Indonesian laboratories.

Published

2014-03-31

How to Cite

MALIK, A., SANTOSO, I., YEHUDA, A., FREISLEBEN, S. K., WANANDI, S. I., HUBER, H., LUTHFA, Z., SALEH, R., & FREISLEBEN, H.-J. (2014). Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia. Microbiology Indonesia, 8(1), 3. https://doi.org/10.5454/mi.8.1.3

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