Cultivation Process Optimization Of Recombinant Bacillus Subtilis Apoptin

Authors

  • MUHAMAD SAHLAN Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
  • YUKI DESIANDINI Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
  • MUHAMMAD IQBAL Chemical Engineering Department, Faculty of Engineering, Universitas Indonesia, Depok 16424, Indonesia
  • NUNUK WIDHYASTUTI Research Center for Biology, Indonesian Institute of Sciences (LIPI), Cibinong 16911, Indonesia
  • AMARILA MALIK Facultry of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • SISWA SETYAHADI Center for Bioindustrial Technology, Agency for Assesment and Aplication of Technology (BPPT), Jalan MH Thamrin 8, Jakarta 10340, Indonesia

DOI:

https://doi.org/10.5454/mi.7.4.3

Keywords:

aeration, agitation, apoptin, optimization, xylose

Abstract

The success of recombinan apoptin production in native form in the previous results open the way to develop this anticancer protein production to the larger scale. We optimized cultivation process of recombinant Bacillus subtilis 168 harbouring pOXGW12His8Arg with apoptin gene in a stirred tank fermentor and shake flasks. The parameters to optimize cultivation process are xylose-inducer concentration, agitation speed, and aeration rate. The xylose-inducer concentration variations are carried out in a shake flasks with 100 mL volume broth, while the agitation speed and aeration rate variation is carried out in a stirred tank fermentor with 3 L volume broth. The xylose concentration is varied between 0-5% w/v, while agitation speed and aeration rate are varied between 150-250 rpm and 0.5-1.5 NL min-1 respectively. The best condition in this cultivation is 1% w/v of xylose, 250 rpm of agitation speed and 1,5 NL min-1 of aeration rate giving the specific growth rate value for each parameter of 0.628 h-1; 0.630 h-1; and 0.747 h-1 respectively. The recombinant apoptin were purified by Ni-NTA column using AKTA system. However, the results showed that the optimum condition of producing recombinant apoptin was 1% w/v of xylose, 250 rpm of agitation speed, and 0.5 NL min-1 of aeration.

Published

2014-04-24

How to Cite

SAHLAN, M., DESIANDINI, Y., IQBAL, M., WIDHYASTUTI, N., MALIK, A., & SETYAHADI, S. (2014). Cultivation Process Optimization Of Recombinant Bacillus Subtilis Apoptin. Microbiology Indonesia, 7(4), 3. https://doi.org/10.5454/mi.7.4.3

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