Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation

Authors

  • SARA GUSTIA WIBOWO 1. BPPT 2. Indonesian Society for Microbiology
  • IS HELIANTI
  • ANI SURYANI
  • BUDIASIH WAHYUNTARI

DOI:

https://doi.org/10.5454/mi.10.3.5

Keywords:

Bacillus halodurans CM1, optimization production, RSM, xylanase

Abstract

A two level factorial design was performed to optimized xylanase production by alkalothermophilic Bacillus halodurans CM1 using response surface method. The variables involved in this experiment were carbon (X), 1 nitrogen source (X) concentration, and pH (XCorn cob and fish powder were use as carbon and nitrogen source 2 3 respectively. Statistical analysis of the experimental results in the range studied, only carbon source gave significant effect on xylanase production.  A second-order model was proposed to represent the enzyme activity as a function of xylan concentration (X) and pH (X).  The optimum corn cobs concentration was 4.37% (w/v), 1 3 fish powder P concentration was 1.75% (w/v) and pH 9. These conditions were tested and validated experimentally since the maximum growth rate achieved with these parameters, and the highest xylanase activity.

Published

2016-09-30

How to Cite

WIBOWO, S. G., HELIANTI, I., SURYANI, A., & WAHYUNTARI, B. (2016). Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation. Microbiology Indonesia, 10(3), 5. https://doi.org/10.5454/mi.10.3.5

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